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Description
Human ADAM33 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against A disintegrin and metalloprotease 33 (ADAM33). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of A disintegrin and metalloprotease 33 (ADAM33) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human A Disintegrin And Metalloprotease 33 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | disintegrin and metalloproteinase 33 (ADAM33) is an enzyme encoded by the ADAM33 gene. It is a member of the ADAM (disintegrin and metalloproteinase domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrin and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein associated with asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 1094 reviews
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Product Reviews
★★★★★ 1
Better off saving your money for a quality brand like Acer or Samsung
If you were looking for a decent gaming monitor with a good refresh rate, you better keep looking. Honestly, I wanted to really like this thing. For $120, it ticked off a lot of great features for me: at least 120Hz refresh rate, 1080p quality, and some nifty reticule marks for FPS gamers like myself. And for a year I had no problems with it until a few days ago. The ability to control input's, power, and toggle the reticule features all lie on one singular button behind the monitor. Here lie's the issue; if that power button starts messing up, there is no way to control or turn on/off the monitor. This is exactly what happened on my unit; the power button/control went out and the monitor was basically useless. I contacted Koorui support via email, and all I got was a vague "Did you confirm the button is really broken" response. Replied back that I did, and the manufacturer has ghosted me. Basically, I HIGHLY advise you just save up a few $80 or $100 dollars and get a decent entry level gaming monitor. By going cheap with this one, you will just end up probably wearing out the power/control button and be left with an expensive paperweight. If you are DIY repair savvy, I am sure that replacing the power/control button probably is not that big of a deal. In which case, go right ahead as it is a decent monitor for the price. But with that said, having this thing break leaves you with basically no options to get it fixed and good luck trying to get a hold of Koorui for support. Sadly, this is a growing trend on buying electronic's here on amazon from smaller brand sellers.
*Update* So as of writing this review, I got Koorui to reach out to me. They would agree to replace the monitor for free, but I would have to front the shipping costs. They have their operations out in California, so if you live on the east coast then expect to pay around $40-50 for shipping for the monitor all the way out there (nearly half the monitor's value). I told Koorui that this just did not make any financial sense and they offered to refund me 50% of the monitor's value. However, they have yet to specify how they will send the refund, so I am unsure of whether I will see my partial refund when this is all said and done. So know that if you do proceed to get this monitor and need to return it past the 30 day return window covered by Amazon. TLDR: just save up an extra $50-100 for a big brand gaming monitor and save yourself the headache of dealing with all of this.
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Reviewed in the United States on October 24, 2024
★★★★★ 5
I feel fully enveloped in the work
Changed my work at home experience. I feel fully enveloped in the work. I don't use it for gaming so can't comment on that. But for laptop heavy work days, this is a great experience.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Perfect for me and my kids
KOORUI 27 Inch Curved Monitor and I am extremely satisfied with its performance. This monitor is perfect for both gaming and desktop use, delivering an immersive and visually stunning experience.
First and foremost, the 27-inch curved display is a game-changer. The curved design enhances the viewing experience, making it feel more engaging and immersive. Whether I'm gaming or working on desktop tasks, the curved screen pulls me into the action and provides a wider field of view.
The FHD 1080P resolution ensures crisp and clear visuals, allowing me to enjoy games and media with stunning detail. The colors are vibrant and accurate, and the overall picture quality is impressive. I can easily spot even the smallest details in games, giving me a competitive edge.
With a 75Hz refresh rate, the monitor delivers smooth and fluid visuals, minimizing motion blur and ensuring an enjoyable gaming experience. Whether I'm playing fast-paced action games or watching videos, the smoothness of the display adds to the overall immersion.
The monitor offers multiple connectivity options, including HDMI and VGA, allowing for easy connection to various devices. I appreciate the flexibility this provides, as I can connect my gaming console, PC, or other devices without any hassle.
One feature that I particularly value is the tilt adjustability. The ability to tilt the monitor allows me to find the perfect viewing angle for maximum comfort, reducing strain on my neck and eyes during long gaming or work sessions.
Another important aspect of this monitor is the eye care technology. The 1800R curvature and eye care features help reduce eye strain and fatigue, even during extended usage. This is a crucial consideration for me, as I spend a significant amount of time in front of the screen.
The sleek black design of the monitor adds a touch of elegance to my setup. It blends seamlessly with my gaming or office environment and enhances the overall aesthetics.
In terms of value for money, the KOORUI 27 Inch Curved Monitor is an excellent choice. It offers a combination of immersive visuals, smooth performance, and eye care features at an affordable price point.
Overall, I highly recommend the KOORUI 27 Inch Curved Monitor for both gaming and desktop use. Its curved display, FHD resolution, smooth refresh rate, and eye care features make it a standout choice. Whether you're a gamer or someone who spends hours working on a desktop, this monitor will undoubtedly enhance your experience."
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Reviewed in the United States on July 18, 2024
★★★★★ 5
I like
Large, tilt. I like. Just does raise up. Goes good with my stand up desk.
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Reviewed in the United States on April 25, 2026
★★★★★ 5
Excent price / quality relation
I had some good expectations about this screen and they were accomplished. The quality is good, it has few modes in its configuration for eye saving or performance.
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Reviewed in the United States on June 3, 2024