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Description
Mouse GLUT9 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a glucose transporter 9 (GLUT9) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the glucose transporter 9 (GLUT9) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Glucose Transporter 9ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glucose transporter 9 (GLUT9) is a protein encoded by the SLC2A9 gene. This gene encodes a member of the SLC2A facilitatory glucose transporter family. Members of this family play an important role in maintaining glucose homeostasis. The encoded protein may play a role in the development and survival of chondrocytes within the cartilage matrix. Two transcript variants encoding different isoforms of this gene have been identified. SLC2A9 has also recently been found to transport uric acid, and genetic variants of this transporter are associated with an increased risk of hyperuricemia, gout, and Alzheimer's disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenate, cell lysate, and cell culture supernatant |
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Product Reviews
★★★★★ 5
Best organizer with a water spout to keep them counter dry!
Color: Black - 9.25″, Color: Black - 9.25″
I am thoroughly impressed with this product. I tried a product that goes over the middle of the sink and it blocked everything off and would always fall off in a funny ways. I had no room for my pots and pans and started getting irritated with it. This is up and out of the way has a little spicket to drain off the water and as you can see I got all of my sponge and soaps in the same spot which is wonderful cleaned off my counter beautifully. I chose the color black to blend in with my black countertop and also to look nice and clean on the counter. One of the best organizers I have ever found for a countertop. It's sturdy metal so far seems rust resistant, but I haven't had it long enough to know for sure. Mobilize easily to clean. Fabulous little sink caddy. I highly recommend to those of you that like to be organized and keep your counter nice and dry, and still arrange things so that it can be out of the way.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Excellent, all around caddy!
Color: Black - 9.25″
The Cisily Sink Caddy is working out nicely.
It holds my sponges, scrubbers and soap bottles. It’s easy to clean and it Excellent all around is well made. I am very happy with my purchase!
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Reviewed in the United States on May 11, 2026
★★★★★ 5
No Rust, No Fuss, A simple but great Sink Item
Color: Black - 9.25″
For years I just had my sponges just laying on or around the sink but this year I decided I needed something to hold the soups and cleaning detergent. Along with not being too pricy and that is how I stumbled upon this sink caddy it has worked amazingly. It holds the detergent, sponge, and tube brushes with no problem and any water is drained out from the little spout underneath it.
You can adjust the dividers so you can have how you want things separeted from each other and with the bottom being plastic you don't have to worry about getting any rust there. If you need something to make more space around your sink this item will help you.
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Reviewed in the United States on May 27, 2026
★★★★★ 4
Functional neat and worth it!
Color: Navy Blue - 9.25″
This caddy has been even more useful than I expected. It instantly made my kitchen look cleaner and more organized compared to having wet sponges sitting on the sink. My counters look much tidier now, and the sponges dry out much better. It may seem like a small addition, but it really makes a big difference.
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Reviewed in the United States on May 9, 2026
★★★★★ 5
Makes the Sink Area All the More Organized
Color: Silver With Towel Rack - 9.25″, Color: Silver With Towel Rack - 9.25″
At first glance I'm sure most people might think this kitchen accessory is kind of silly; a caddy for your sponge and dish soap? But honestly, it makes the kitchen area look at the more organized and keeps things in place. I sprung for the model with the towel holder because I always feel weird about bacteria sitting in the wet microfiber cloth that I use for cleaning the kitchen countertop and my dining table. The little dowel allows it to dry very quickly.
The holder can be configured in a few different ways to accommodate your cleaning items. I like that it's wide enough to comfortably hold the large bottle of dishwashing soap I buy and it's the perfect width for Sponge Mommy/Daddy sponges.
I've had it less than a week and haven't had to wash it yet, but it seems like it will hold up well in the dishwasher and won't rust. Since I don't have any sponges on a handle, I may use the removable compartment for those (that you can see on the left in my photo) for other kitchen tools.
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Reviewed in the United States on April 7, 2026