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Description
Mouse HDL3 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a high-density lipoprotein 3 (HDL3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of high-density lipoprotein 3 (HDL3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse high-density lipoprotein 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | High-density lipoprotein (HDL), a serum protein, is a complex lipoprotein composed of lipids, proteins, and the regulatory factors they carry. It is also known as a1 lipoprotein. HDL is primarily synthesized in the liver and small intestine. Nascent HDL synthesized in the liver is primarily composed of phospholipids and ApoA I. Under the action of LCAT, free cholesterol is converted into cholesterol esters, and the lipoprotein becomes mature globular HDL3, which is then converted into HDL2 through the action of LPL. LCAT completes the conversion of nascent discoidal HDL into HDL3 and HDL2 through transesterification, reducing the concentration of free cholesterol in plasma HDL, establishing a concentration gradient for cholesterol to flow from cell membranes to plasma lipoproteins, and reducing tissue cholesterol deposition. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
Based on 518 reviews
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Product Reviews
★★★★★ 4
Great value and great monitor
Size: 27"/QHD/200Hz
As a note to new buyers: a chemical-like odor may be emitted from your monitor's exhaust for the first 2-3 weeks--at least mine did. I did some research and this was likely attributed to some cleaning chemicals from the factory, and it is burning off from the heat of the monitor. Simply have good ventilation in your room, and the odor should dissipate in 2-3 weeks. I've had this monitor for over a year now, and there is no odor anymore.
Now, for the actual review: This monitor is great quality for the price! Not perfect, as I've found this monitor doesn't display smooth gradients of dark very well. For example, when I'm in a dark room in a video game, the dark ambient colors show slightly pixelated rather than smoothly blended.
Other than that, this monitor works like a charm! Colors are great, and monitor settings are easily adjustable. One complaint I have is that I can't change the monitor's brightness using the Windows OS' brightness settings--you have to change the brightness/contrast using the monitor's buttons.
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Reviewed in the United States on December 2, 2024
★★★★★ 5
Great quality
Size: 27" 240hz/FHD
I bought this for my son as a second monitor. Graphics are sick! Feels like you're really in the game.
My son absolutely loves it.
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Reviewed in the United States on April 25, 2026
★★★★★ 5
A great value for a state of the art monitor.
I use two of these at my home work station. I am not a gamer, I use these mainly for working on multiple spread sheets and financial programs. The high pixel count of these 8k video displays makes it easy to read the screens. I have Windows 11 on my computer, so installation of the 3 monitors was easy. I have my computer monitor set at 150% and these two monitors set at 125% magnification. All three are at eye level. They came with display port cables which my computer and hubs do not have, so I in a 8K HDMI cable for each. I do play a few videos, and those look great. The curved screen helps in keeping all the monitor sort of equidistant from my eyes which helps everything on the 3 screens stay in focus for my 80 year old eyes. I have reflections on my computer screen, but both of these Koorui monitors are reflection free.
I first went to Costco to pick up a monitor and this allowed me to zero in that I wanted the 8k and curved screen in a 24 or 27 inch monitor. They did not have that combination. I tried just one of the Koorui monitors along with my older monitor. The Koorui was so far superior that I quickly purchased the second one and tossed out my outdated one. I use these for hours each day, and at this low price I feel I can easily justify this investment. I feel secure these monitors will be with me for a long time.
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Reviewed in the United States on October 27, 2023
★★★★★ 1
This sucks.
Well i picked this up on sale for black friday and finally got around to installing my new video card, psu, extra ssd, and finally hooking up this new monitor. The new video card doesn't have a spot for the cable from my old 1080p monitor, so this is the only monitor i can use. It's christmas eve btw.
Anyways the monitor fires up fine and looks great for about 6 hours of use. Then as i'm getting ready to boot up elden ring a big vertical magenta line of pixels appears on the right half of the screen, as well as a grey horizontal line of pixels. I didn't do anything before this happened, the monitor was sitting completely still on my desk. One second it worked the next i've got this line.
I tested another cable, and while unplugging the displayport cable from the monitor the pink line stayed there as it was registering "no input." So it's definitely the monitor that's broken and not something wrong with my video card or software. I tried contacting technical support early in the morning and still haven't received anything at 5 pm.
I realize it's christmas eve but I'm now stuck without a functioning monitor for probably my entire time off for christmas, and will have to pay return shipping, which soaks up all the money i saved getting it on sale.
Over all i'm very unhappy with this purchase.
edit: Koorui, the manufacturer, never got back to me about the broken monitor they sent me, so i contacted amazon and told them my situation. they told me they couldn't send a replacement because it wasn't available, even though you can order the exact monitor off amazon through prime right now. this order was a critical failure in service with both koorui and amazon. they can't seem to lift a finger for someone who paid them money for a product they sell.
they want me to ship the monitor back, receive my money back, then purchase the same monitor for a higher price from them. I'm changing my review to 1 star and i would give 0 stars if i could. unbelievable.
edit: ok i shipped the monitor back. they sent me a replacement monitor and this monitor also does not work. it doesn't turn on. i plugged it into both a surge protector and a power strip, then switched the cable and it doesn't turn on. i would be extremely wary of this company and their products.
edit 2: by far my worst purchasing experience on amazon. with the second broken monitor they shipped me the support guy actually asked if i would take it to a tv repair shop. i told him we don't have that in the united states and returned the item for a full refund. they got to keep my $150 for about 3 months though. these people should not be allowed to sell on amazon.
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Reviewed in the United States on December 24, 2022
★★★★★ 5
Gaming
Size: 27"/QHD/200Hz
Idk about the specs but my it's used strictly for gaming and got no complaints.
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Reviewed in the United States on June 10, 2026