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Description
Vimentin Recombinant Rabbit mAb (SDT-029-20)Product Specification Host Rabbit Antigen Vimentin Immunogen Synthetic Peptide Accession P08670 Clone Number SDT 029 20 Antibody Type Rabbit mAb Isotype IgG Application WB, IHC P, ICC, IF Reactivity Hu, Ms, Rt Predicted Reactivity Pr, Bv, Cz, Pg, Hm, Av Purification Protein A Research Area Neuroscience Concentration 1 mg ml Molecular Weight 56kDa Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03%
Product Specification
| Host | Rabbit |
| Antigen | Vimentin |
| Immunogen | Synthetic Peptide |
| Accession | P08670 |
| Clone Number | SDT-029-20 |
| Antibody Type | Rabbit mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Pr, Bv, Cz, Pg, Hm, Av |
| Purification | Protein A |
| Research Area | Neuroscience |
| Concentration | 1 mg/ml |
| Molecular Weight | 56kDa |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:1000 | |
| ICC | 1:200 | |
| IF | 1:200 |
Background
Vimentin is a structural protein that in humans is encoded by the VIM gene. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
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