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Description
Human Colorectal cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath |
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| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Very good story!!!
Format: Kindle
I loved the characters and the storyline. Even a little mystery weaved in to the RH experience. The story made me laugh, cringe, hold my breath, and get sweaty. The love scenes were 🔥🔥🔥 🔥!!!! Great story to read!
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Reviewed in the United States on January 8, 2026
★★★★★ 4
So So Amazing
Format: Kindle
I received this book wiht the understanding that I could leave a voluntary and honest review.
In this book we meet June Wilder, Rhett Lawson, Cole Thibault, & Elias Nystrom. When this book opens we find that June has just joined the Atlanta Reapers as their new physical trainer. From the moment she starts she knows that she is going to get push back but there is something about not only this team but these guys that have become more to her than she thought when she first started. Rhett is the kind of guy who make a joke with anyone. One night when something happens with June he realizes there is something more than just jokes with her. Cole has been compartmentalized his life for years now. With the addition of June to the team he finds himself finding a person who understands his compartments and lets him be him. Elias is a man who is a an island. When he learns that opening up to one person who sees him allows him to grow and heal in many ways. Can they all find a HEA or will their positions make that impossible?
Another amazing story by this author. I would highly recommend it to anyone.
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Reviewed in the United States on December 31, 2025
★★★★★ 5
Good spicy campy hockey
Format: Kindle
I really enjoyed the character development. Reading about their growth and dealing with individual issues made good entertainment. Some surprising twists that made the book even better. Really glad we didn't have to go down the angst road where the public got to damage the budding relationships.
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Reviewed in the United States on January 1, 2026
★★★★★ 5
Great read!
Format: Kindle
Wonderful characters with great humor, a bit of mystery and a strong silent grumpy Sentinel to stand with the leading lady. They make her stronger but she also makes them stronger. I love the dynamics and the way it played out. This was a fun read!.
I received a free copy of this book via Booksprout and am voluntarily leaving a review.
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Reviewed in the United States on December 26, 2025
★★★★★ 5
June lives her life squarely in the NSFW zone...
Format: Kindle
June Wilder has embarked on the career of a lifetime-- the newest head physical trainer for NHL's Atlanta Reapers! She's relatively young for the position, not to mention female. In a male dominated sport, she's hoping to one day earn the trust and respect of the players, coaching staff and the organization. What she doesn't expect is to be an equal participant in flirty banter and NSFW activities in her office with handsome Right Wing Rhett Lawson. She quickly finds that Team Captain Cole Thibault is a confident and calming presence in her daily work activities-- and the man she goes on quite possibly the most perfect date with. Then there's Elias Nystrom, the Swedish Goalie who is quiet, HULKING in size, and downright frightens her-- with whom she shares a secret which could get her in hot water if discovered. Each of these team members intrigues her. Each she has a relationship with that is not entirely permitted as their head physical trainer. As she gets to know each of them better, she's falling deeper for each of them. Will she successfully navigate through the season without losing not only her job but her heart? What happens when each of the men find out about each other? Will she be asked to choose between them?
This book has everything I look for in a sinfully decadent read-- a strong, independent, and likable FMC; charming, protective, and sexy MMCs; a plot that is entertaining, well thought out and executes some unexpected twists; and has chemistry that explodes off of the pages. Once again author Cassie Cole has expertly achieved all of these things in her latest book "Hat Trick". I'm a big fan of the RH genre. Of all the authors I read, Cassie Cole is far and away my favorite to date. If you read this book, you will not be disappointed!
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Reviewed in the United States on December 1, 2025